Abstract submitted to the International Europhysics Conference on High Energy Physics July 12 - 18, 2001, Budapest, Hungary _________________________________________________________________________ Measurements of jet substructure in neutral current deep inelastic scattering and determination of alpha_s at HERA ZEUS Collaboration Abstract The substructure of jets has been measured in neutral current interactions at Q^2 > 125 GeV^2 with the ZEUS detector at HERA using an integrated luminosity of 42.7 pb^{-1}. Jets have been identified in the laboratory frame using the longitudinally invariant k_T-cluster algorithm. The substructure of jets has been studied in terms of the jet shape and subjet multiplicity. The subjet multiplicity is defined as the number of clusters resolved in a jet by reapplying the jet algorithm at a smaller resolution scale. Measurements of the integrated jet shape and mean subjet multiplicity for jets with transverse energies E_T^jet > 14 GeV are presented. The jets are observed to become narrower and the mean subjet multiplicity decreases as E_T^jet increases. Next-to-leading-order QCD calculations give a good description of the measurements and are used to make a determination of alpha_s.